source bacillus sp strain atcc 27860 indicator strain  (ATCC)

Journal:

Article Title: Genes Specific for the Biosynthesis of Clavam Metabolites Antipodal to Clavulanic Acid Are Clustered with the Gene for Clavaminate Synthase 1 in Streptomyces clavuligerus

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Production cultures were grown under conditions similar to those used for seed cultures. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Reference or source Strains Bacillus sp. ATCC 27860 Indicator strain; sensitive to alanylclavam on minimal medium 26 E. coli ER1447 ara-14 F − dam-13 ::Tn 9 (Cm r ) dcm6 fhuA31 galK2 galT22 hisG4 hsdR2 (r k − m k + ) lacY1 leuB6 mcrA mcrB1 mtl-1 rpsL136 (Str r ) supE44 thi-1 tsx-78 xyl-5 J. McCormick, Harvard University, Cambridge, Mass.

Techniques: Plasmid Preparation, Northern Blot, Clone Assay, Isolation, Modification